The aim of the present study was to determine the combined effect of both stress and EFA deficiency on several biological and biochemical parameters. Fish were fed during 15 weeks two isocaloric and isoproteic diets: a control diet based on fish oil and formulated to meet the n-3 HUFA requirements for this species 1. Each experimental diet was evaluated both at high and low stocking densities 10 and 3. High stocking density produced a chronic stress situation with elevation of plasma cortisol levels.
Broilers stocked in high densities may be prone to oxidative and inflammatory insults, resulting in impaired health status, growth performance, and meat quality. Growth performance was recorded weekly. Blood and tissues were sampled at the end of each period. Those differences were partially attenuated by the extra methionine supplementation. The interaction effect was also found on activities of glutathione peroxidase and superoxide dismutase in the thigh of growers. Modern broiler houses are designed to raise the animal flocks at relatively high densities to save space and revenue. However, broilers raised under these conditions may suffer from stocking density stress.
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